The Binding of Isaac: Afterbirth is the expansion to The Binding of Isaac: Rebirth. The DLC was released on October 30th, 2015 and costs $10.99. It was released on May 10, 2016 on Xbox One and PS4.

For a list of the pre-release blog posts, see The Binding of Isaac: Afterbirth (Pre-Release).


Other changes


95 new items were added in the expansion: 19 activated items and 76 passive items.

Activated Collectibles

Name Icon Description Recharge
Box Of FriendsBox of Friends Icon.pngUpon use, duplicates all of Isaac's familiars for the current room.4
D1245x45pxRe-rolls all rocks in the current room into other random objects, such as bomb rocks, metal blocks, TNT, or poop.3
D745x45pxRestarts the current room and brings back all enemies, allowing more rewards to be acquired by completing the room.3
D845x45pxRe-rolls all of Isaac's stats.4
Diplopia45x45pxSingle-use. Duplicates all pickups and item pedestals in the room.none
Friendly Ball45x45pxThrowing the ball at an enemy instantly kills it and captures it. The captured enemy can later be released and will be charmed for the duration of the room.3
Glass Cannon45x45pxWhen used, reduces Isaac's health to half a heart (including soul and black hearts) and fires one large piercing spectral tear that does a lot of damage. The damage done by Glass Cannon scales with Isaac's damage (at base damage it does 45 damage).5 seconds
Glowing Hour Glass45x45pxWhen used, this item will rewind time, put Isaac back in the previous room, and undo anything that happened in the current room. Any health, pickups, or items gained or lost will be reset back to the previous state.2
Jar Of Flies45x45pxEvery time Isaac kills an enemy, a fly will be added to the jar, up to a maximum of 20. Using the Jar of Flies will release all the flies that are currently in the jar as Blue Flies that will damage enemies.none
Kidney Bean45x45pxUpon use, Isaac farts, charming all nearby enemies.2
Mega Bean45x45pxWhen used, the Mega Bean will freeze all enemies in the current room for a couple of seconds, deal 5 damage and poison any enemies near Isaac, and send a wave of spikes that deals 10 damage across the room in the direction Isaac is facing.3
Mega Blast45x45pxUpon use, Isaac fires a laser similar to the one used by Mega Satan. This laser deals massive damage, lasts for approximately 15 seconds, and persists between rooms. Isaac is pushed backwards by the force of the laser while it is active.12
Mine Crafter45x45pxWhen used, places an explosive TNT barrel that can be pushed and used to destroy rocks or damage enemies. If used a second time in the same room while the previous TNT barrel is still intact, it will remotely explode the TNT.1
Mom's Box45x45pxWhen used, Mom's Box will drop a random trinket on the ground. While held, increases luck by 1 and doubles the effects of trinkets.4
Placebo45x45pxWhen used, copies the effect of the pill Isaac is currently holding without consuming it. Does not copy the effects of cards or runes.4
Tear Detonator[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]When used, will detonate any tears currently on the screen and cause each one to split into 6 more tears which will fire in a circle, similar to Tammy's Head.1
Teleport 2.0[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]When used, teleports Isaac to a random room that has not been explored yet. If all rooms are explored, including secret rooms, it will teleport Isaac to the Devil or Angel room. If all rooms are explored, including the Devil or Angel room, it will teleport Isaac to the I AM ERROR room. If used in a room with enemies, it will teleport Isaac out and back into the exact same room.4
Ventricle Razor[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Can be used to place an orange trapdoor and a blue trapdoor. Upon entering a trapdoor, Isaac is teleported to the other trapdoor.none
Wooden Nickel[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]When used, has a 50% chance to drop one random type of coin (penny, nickel or dime)1

Passive Collectibles

Name Icon Description
八寸钉8 Inch Nails Icon.png增加+1.5点伤害,以撒眼泪变为钉子,增加击退。
祭典之刃Athame Icon.png角色受到伤害后,在身边产生一个黑环,对敌人造成接触伤害。
背叛Betrayal Icon.png角色受到伤害后,对房间内所有敌人造成魅惑效果,使它们互相攻击。
奶嘴Binky Icon.png增加0.7点射速,给予1个魂心,角色体型缩小。
黑粉末Black Powder Icon.png在角色身后留下一道黑色粉末。使粉末闭合成圆将在地上生成一个五芒阵,在行走在内的敌人产生每帧10点的伤害。
炸弹人Bomber Boy Icon.png给予5个炸弹。角色放置的炸弹呈大十字型爆炸(大约为5个炸弹的爆炸范围)。笑脸炸弹的爆炸范围同样受影响。
笨笨Bumbo Icon.png生成一个乞丐跟班,在房间中移动并拾起金币。每6个金币会让笨笨进化到下一阶段,从三阶进化到四阶则需要12金币。二阶笨笨会长出身体,在房间里爬行。三阶笨笨会发射与人物同一方向的眼泪。四阶笨笨不再发射眼泪,会缓慢地追赶敌人并造成接触伤害,随机掉落炸弹。同时,四阶笨笨会持续捡起硬币,生成基础掉落。
爆破袋Bursting Sack Icon.png蜘蛛不再攻击角色,或对角色造成伤害。类似粪臭素。
恶魔胚胎Cambion Conception Icon.png角色受伤一定次数后给予一个恶魔跟班。恶魔跟班可能为:黑暗乞丐,恶魔宝宝,水蛭,硫磺火宝宝,魅魔,及夜魔。不能给予多过4个恶魔跟班。
车载电池Car Battery Icon.png主动道具双倍效果。
CenserCenser Icon.pngGives Isaac a golden censer familiar which creates a huge aura of light that slows down any enemies inside it.
ChaosChaos Icon.pngChaos causes items to be chosen from random item pools. When picked up, drops between 1-6 random pickups on the floor.
Charged BabyCharged Baby Icon.pngA familiar baby that drops a battery every 4 rooms. Has a small chance to freeze all enemies in the room when hit.
Circle Of ProtectionCircle of Protection Icon.pngGives Isaac a large white halo around him, that deals a bit of damage to any enemies in contact with the ring. Every time an enemy bullet enters the ring, there is a chance it will reflect a homing tear back at the enemy.
ContinuumContinuum Icon.png+2.25 Range Up. +1.5 Tear Height. Isaac's tears travel through walls and appear out of the opposite wall. Isaac's tears also travel over rocks and objects.
Crack JacksCrack Jacks Icon.pngAdds one heart container and drops a random Trinket.
Crown Of LightCrown Of Light Icon.pngGives Isaac a crown that glows blue when active, and turns gray when inactive. While Isaac has no empty red heart containers, Isaac's tears are replaced with blue diamond tears that deal double damage. Regardless of health, the crown will deactivate for the current room when Isaac takes damage.
Curse Of The TowerCurse of the tower Icon.pngCauses Isaac to spawn 6 troll bombs on the floor every time he gets hit, similar to the Anarchist Cookbook mechanic.
Dead Eye45x45pxIsaac's damage increases each time an enemy is hit. Damage is reset upon missing a shot.
Deep Pockets45x45pxAllows Isaac to carry two cards or pills (or a combination of both) at the same time.
Eden's Blessing45x45pxTears up. Grants a random item at the start of the next run.
Empty Vessel45x45pxWhile Isaac has no red hearts, this item grants flight and a random chance to activate an invincibility shield for a few seconds. If Isaac gains any red hearts, these benefits will disappear.
Epiphora45x45pxShooting in one direction increases Isaac's rate of fire until the cap is reached. As soon as Isaac changes direction or stops shooting, rate of fire is reset to its normal amount.
Evil Eye45x45pxWhile firing tears, Isaac has a chance to fire an eye across the screen with a very slow shot speed, which will fire tears of its own in the same direction as Isaac. The eye will be destroyed if it comes into contact with an enemy or obstacle.
Explosivo45x45pxIsaac's tears have a chance to stick to enemies and explode.
Farting Baby45x45pxA familiar that follows Isaac and blocks tears. If a tear hits Farting Baby, there is a high chance for it to fart, which will either knock back, poison, or charm all nearby enemies.
Fate's Reward45x45pxA blue head familiar that will follow Isaac and fire tears copying his tear effects and damage.
Friend Zone45x45pxA white orbiting fly which deals 3 contact damage per tick to enemies. This familiar orbits Isaac at a middle length distance somewhere between Distant Admiration and Forever Alone.
Fruit Cake45x45pxIsaac's tears gain a random effect that changes every shot.
GB Bug45x45pxA glitch familiar that bounces around the room and applies a random status effect to any enemies it comes into contact with and has a chance to re-roll pickups that it passes over.
Glitter Bombs45x45pxGives Isaac 5 bombs, and makes Isaac's bombs have a chance to drop random pickups or charm enemies when they explode.
God's Flesh45x45pxTears now have a random chance to apply a shrinking effect, causing enemies to shrink in size and also run away from Isaac. Shrunk enemies can be crushed and killed by walking over them.
Head Of The Keeper45x45pxIsaac's tears become coins and now have a chance to drop pennies on the floor upon successfully hitting an enemy.
Holy Light!45x45pxIsaac has a random chance to fire a Holy tear, which when it hits an enemy, will spawn a Crack The Sky style light beam on the same enemy, dealing damage.
Host Hat45x45pxA host familiar that sits on Isaac's head and has a random chance to block enemy shots and retaliate with 3 tears. Grants immunity to explosions.
Immaculate Conception45x45pxUpon picking up a certain amount of health, spawn a permanent angelic familiar.
Incubus45x45pxA demonic familiar that follows Isaac and shoots tears identical to Isaac's in terms of damage, range, fire rate, and effects.
Key Bum45x45pxSpawns a portable Key Master that follows Isaac and collects keys, giving random chests in return.
Kidney Stone45x45pxRandomly while firing tears, Isaac will stop firing and turn red, where he will charge and release a lot of 'tears' in one go. In the burst of tears is a kidney stone which deals a lot of damage.
Lil' Chest45x45pxSpawns a chest familiar that follows Isaac and drops a random pickup every few rooms.
Lil Gurdy45x45pxGives Isaac a Gurdy familiar that will charge around the room dealing contact damage to enemies. Lil Gurdy is charged by holding down a fire button and letting go. The longer the button is held, the faster Lil Gurdy will fling itself across the screen. Lil Gurdy can pass over objects and obstacles in the room.
Lil' Loki45x45pxA Loki familiar that follows Isaac and shoots 4 tears in a cross pattern.
Lost Fly45x45pxA familiar fly that moves in a straight line and attaches itself to the first obstacle or wall it comes into contact with in each room. If any enemies walk into its path, it will deal 7 contact damage per tick. If the obstacle it is attached to is destroyed then it will continue rolling in the direction it was previously travelling until it meets another wall or obstacle.
Lusty Blood45x45pxEach time Isaac kills an enemy, Isaac's damage increases for the current room.
Marked45x45pxIsaac will now fire tears automatically directed at a red target on the ground which is controlled by the player. This allows for a full 360 degree tear firing radius. Can be overridden by other tear modifiers such as Mom's Knife or Brimstone.
Maw Of The Void45x45px+1.0 Damage Up. After continuously firing tears for 3 seconds, a red cross appears on Isaac's head that, upon releasing the fire button, creates a black ring which deals a lot of damage to any enemies inside it. The ring rapidly deals damage equal to Isaac's tear damage to any enemies in contact with it.
Milk!45x45pxA glass of milk follows Isaac and spills on the floor upon taking damage. After the milk spills, Isaac gains a tears up that lasts for the rest of the room.
Mom's Pearls45x45px+1.25 Range Up. +0.5 Tear Height. +1 Luck Up.
More Options45x45pxTwo items spawn in every Treasure Room. Only one can be taken; the other will disappear.
Mr. Dolly45x45pxTears and Range up. Spawns 3 random types of hearts on the floor when picked up
Multidimensional Baby45x45pxSpawns a familiar that will follow Isaac's movements on a 2.5 second delay. Tears that pass through the baby will be doubled and increase in damage.
My Shadow45x45pxEach time Isaac takes damage, inflicts Fear on all enemies in the room and spawns a black Charger familiar that will attack enemies. The Charger will be killed if it is damaged too much.
Night Light45x45pxA cone of light shines in the direction Isaac is facing that slows enemies and enemy shots.
No. 245x45pxContinuously firing tears for several seconds causes Isaac to drop a Butt Bomb. Butt Bombs explode for 50 damage like normal bombs, but also deal 10 damage to all enemies in the room and daze them for a short period of time.
Papa Fly45x45pxA blue fly familiar follows Isaac's movements on a 3 second delay. Papa Fly will fire tears at nearby enemies.
Pay To Play45x45pxThis item turns all doors that require a key into doors which need a coin to enter instead. This affects shops, item rooms, libraries and other key doors.
PJs45x45pxAdds 4 Soul Hearts and fully restores Isaac's red health.
Pupula Duplex45x45pxTransforms Isaac's tears into a wide arc shape which gives them a much larger hitbox. Gives Isaac spectral tears which allows them to travel through objects in the environment (i.e. rocks).
Purity45x45pxThis item will boost one of Isaac's stats depending on the color of the aura around him. The aura will disappear upon taking damage and will reappear with a random color upon entering a new room. A red aura boosts damage, a blue aura boosts rate of fire, a yellow aura boosts speed, and an orange aura boosts range.
Obsessed Fan45x45pxA purple fly familiar that follows Isaac's movements on a 3 second delay and deals contact damage to enemies.
Restock45x45pxCauses shops to instantly restock their items when they are bought. Granted by default for every character in Greed Mode.
Rune BagRune Bag Icon.pngA bag that follows Isaac and drops a random mystic rune every 3 rooms.
Sack Head[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Greatly increases the chance to find sacks.
Scatter Bombs[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Adds 5 bombs. Isaac's bombs explode into 2-4 tiny bombs.
Seraphim[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]An angel familiar which follows Isaac and fires homing tears which deal 10 damage at -25% shot speed and a reduced fire rate.
Serpent's Kiss[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Tears now have a random chance to apply a poison effect, causing 4 or 6 damage per tick to enemies (Similar to The Common Cold item). Isaac now deals poison damage on contact with enemies similar to The Virus item (Damage over time 4 or 6 per tick). Enemies that were poisoned have a chance to drop a black heart when killed.
Sticky Bombs[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]+5 bombs. Bombs stick to enemies and spawn 2-4 Blue Spiders if they kill an enemy.
Spear Of Destiny[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]In addition to tears, Isaac now holds a spear in front of him which deals damage equal to 2 times his tear damage, and occasionally inflicts Fear. The spear cannot be thrown, but deals damage while it is in contact with an enemy.
Spider Mod[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Displays Isaac's tear damage and enemy health bars. Spawns a gray spider familiar that wanders around and inflicts a random status effect on any enemies it comes into contact with. Occasionally spawns a blue spider upon clearing a room.
Succubus[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Spawns a flying familiar that bounces around the room with a black aura, dealing rapid damage to any enemies inside it. While standing in the aura, Isaac gains a 1.5x tear damage multiplier.
Sworn Protector[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]An orbital angel which does 7 contact damage per tick and blocks enemy shots. When it blocks a shot, it has a very small chance to drop an eternal heart.
Tech X[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Tears are replaced with laser rings that pierce through enemies and deal damage to enemies within them. Can be charged to increase the size of the ring.
The Wiz[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Tears simultaneously fire out of both eyes diagonally, similar to the effect of the R U A Wizard? pill. Isaac's tears also travel above all obstacles.
Toxic Shock[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Upon entering a room, poisons all enemies, dealing double Isaac's tear damage. Killing enemies leaves a small, short lasting pool of green creep on the ground.
Tractor Beam[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Isaac emits a beam from his face. Isaac's tears travel in the path of the beam.
Zodiac[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Zodiac will give Isaac a random zodiac item effect that changes after every floor.


29 new trinkets were added in the expansion.

Item Icon Effect
Blasting Cap[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Exploding bombs have a chance to drop another bomb pickup.
Black Feather[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Increases Isaac's damage for possessing certain evil items.
Blind Rage[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Increases invincibility time after getting hit.
Blister[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Increases knockback of tears.
Brown Cap[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Poop will explode after being destroyed.
Bob's Bladder[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Leaves a pool of green creep under all bombs Isaac places, which damages enemies that walk over it.
Child Leash[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Causes familiars to stay closer to each other.
Cracked Dice[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Whenever Isaac gets hit, there is a chance that the effect of The D6, D8, D12, or D20 gets activated.
Endless Nameless[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]When Isaac uses a pill, card, or rune, there is a chance to spawn another one of the same type.
Error[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Gives a random trinket effect each room.
Faded Polaroid[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Occasionally causes Isaac to blend into the ground, which briefly confuses all enemies in the room.
Golden Horse Shoe[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]+15% chance for a Treasure Room with two items to spawn.
Karma[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Donating to a Donation Machine has a chance to heal Isaac for a full red heart or spawn a Beggar. Increases the chance that donating to a Donation Machine will increase Isaac's luck.
Lazy Worm[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Tear shot speed is greatly reduced.
Lil Larva[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Spawns a Blue Fly from every pile of poop Isaac destroys.
Louse[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Occasionally spawns a Blue Spider.
Mom's Locket[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Grants half a red heart each time a key is used. Converts half red heart pickups into full red hearts.
NO![[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Removes all active items from every item pool.
Poker Chip[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Chests have a 50% chance of paying out with extra pickups and a 50% chance of paying out with nothing or enemies.
Rainbow Worm[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Isaac's tears gain a random worm effect that changes every 3 seconds.
Rib Of Greed[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Prevents Greed and Super Greed from appearing in Shops and Secret Rooms.
Safety Scissors[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Troll Bombs turn into Bomb pickups before they explode. Mega Troll Bombs turn into 1+1 Free Bombs before they explode.
Second Hand[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Increases the time that status effects stay on enemies.
Shiny Rock[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Tinted rocks and rocks with Crawl Spaces will flash white every 10 seconds.
Store Key[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Automatically opens all Shops.
Stud Finder[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Increases the chance to find a Crawl Space.
Super Magnet[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Pulls enemies and pickups towards isaac.
Tape Worm[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Doubles Isaac's range and halves his tear height.
Watch Battery[[File:{{{image}}}_Icon.png|45x45px|class=pixelImage]]Increased chance of batteries spawning. Has a chance to add an extra charge to Isaac's activated item upon completing a room.


Several new bosses were added in the expansion. Additionally, many bosses received new champion variants.

Boss Rag Man.png
Boss Little Horn.png
Boss Dangle.png
Boss Turdlings.png
Rag Man Little Horn Dangle Turdlings
Boss The Frail.png
Boss The Stain.png
Boss The Forsaken.png
Boss Brownie.png
The Frail The Stain The Forsaken Brownie
Boss Ultra Greed.png
Hush Ultra Greed


Many new monsters were added in the expansion. Additionally, several new champion types were added.

Monster Description
Dart Fly
Dart Fly.png
Flies towards Isaac and attempts to mimic his movements. Deals contact damage.
Swarm Fly
Swarm Fly.png
Follows Isaac in large groups and deals contact damage.
Drowned Boom Fly
Drowned Boom Fly.gif
Flies around the room diagonally, dealing touch damage. Upon death, explodes, fires four blood shots in the cardinal directions, and spawns a Drowned Charger.
Corn Dip
Corn Dip.png
Quickly dashes in random directions, dealing contact damage.
Dank Squirt
Dank Squirt.png
Dashes towards the player and deals contact damage. Spawns 2 Clots upon death.
Pops out of the ground in random locations, creating a small rock wave. Fires an explosive Ipecac shot at Isaac if he is in sight.
Pops out of the ground in random locations and spits a blood shot at Isaac or spawns a Dart Fly if Isaac is in sight.
Drowned Hive
Drowned Hive.png
Walks around the room, avoiding Isaac while spawning Drowned Chargers. Spawns a Drowned Charger upon death.
Flies around the room diagonally, occasionally spitting out a Dart Fly.
Flaming Gaper
Flaming Gaper.png
Walks towards Isaac, dealing contact damage. Usually turns into a Pacer or a Gusher upon death.
Walks towards Isaac, dealing contact damage and leaving behind a trail of damaging red creep. Occasionally shoots a blood shot towards Isaac.
Black Bony
Black Bony.png
Wanders around the room and throws three bones in a row at Isaac when he is in sight. Explodes upon death.
Dank Death's Head
Dank Death's Head.png
Moves diagonally around the room, dealing contact damage. Needs to be killed to complete the room.
Flesh Death's Head
Flesh Death's Head.png
Moves diagonally around the room, dealing contact damage. Explodes into 6 blood shots in all directions upon death. Can be killed, but also dies when the room is cleared.
Fat Bat
Fat Bat.png
Slowly flies towards Isaac. Shoots when Isaac aligns diagonally with it and will actively try to avoid Isaac's shots.
Red Ghost
Red Ghost.png
Disappears, then reappears near Isaac and fires a brimstone laser.
Flaming Fatty
Flaming Fatty.png
Slowly walks towards Isaac. Occasionally farts, which pushes Isaac and adjacent enemies away. Has a chance to leave behind a damaging flame upon death.
Conjoined Fatty
Conjoined Fatty.png
Slowly walks towards Isaac. Occasionally spits a line of damaging green creep towards Isaac. Turns into a regular Fatty upon death.
Blue Conjoined Fatty
Blue Conjoined Fatty.png
Slowly walks towards Isaac and occasionally spits a homing Ipecac shot at Isaac.
Dank Globin
Dank Globin.png
Chases Isaac, dealing a full heart of contact damage. Upon taking enough damage, it collapses into a pile of goo, spawns a spider, and leaves a puddle of slowing black creep. Will regenerate after a short period of time if not killed quickly.
Black Globin
Black Globin.png
Chases Isaac, dealing a full heart of contact damage and spawning spiders. Upon taking enough damage, it separates into a head and body. The head slides towards Isaac, leaving a trail of damaging red creep. The body wanders around aimlessly, occasionally spawning spiders.
Black Globin's Body
Black Globin's Body.png
Walks aimlessly around the room, occasionally spawning spiders.
Bone Knight
Bone Knight.png
Moves erratically around the room, charges toward Isaac when in line of sight, and leaves behind a trail of damaging red creep. Can only be damaged from behind.
Mega Clotty
Mega Clotty.png
Slowly moves around the room, occasionally hopping and firing 6 blood shots in all directions. Splits into two Clotties upon death.
Mom's Dead Hand
Mom's Dead Hand.png
Falls from the ceiling. If it lands on Isaac, it deals damage and teleports the player back to the spawn room of the floor. If it doesn't land on Isaac, it spawns several rock waves that travel outwards. Spawns several spiders upon death. Entering a room containing Mom's Dead Hand will warn the player with Mom's laugh.
Drowned Charger
Drowned Charger.png
Slowly moves around the room, charging at Isaac when he gets in its line of sight. Explodes into four blood shots in the ordinal directions upon death.
Scarred Para-Bite
Scarred Para-Bite.png
Moves towards Isaac, dealing contact damage and firing short-range blood shots. Can burrow underground and reappear elsewhere.
Scarred Guts
Scarred Guts.png
Attaches itself to a wall or obstacle and rolls around it, leaving a trail of damaging red creep.
Scarred Double Vis
Scarred Double Vis.png
Charges and fires a Brimstone laser which fires from all four sides when Isaac gets in its line of sight.
Slides around the room, occasionally stopping to spew a fountain of short-range blood shots in all directions or to spit a line of damaging red creep. Spawns a Host upon death.
Slowly drifts towards Isaac, frequently firing 5 blood shots in quick succession towards Isaac. Tries to avoid Isaac's shots and occasionally teleports and reappears elsewhere.
Greed Gaper
Greed Gaper.png
Walks towards Isaac, dealing contact damage.
Blue Gaper
Blue Gaper.png
Walks towards Isaac, dealing contact damage. Has triple the health of a normal Gaper.
Blue Boil
Blue Boil.png
Stationary enemy that decreases in size when hit, but regenerates to its full size until fully destroyed. Fires 1-3 blood shots towards Isaac when fully swollen.
Full Fly
Hush Fly.png
Flies toward Isaac erratically, dealing touch damage. Splits into 6 directional shots upon death.



  • Afterbirth is the placenta and fetal membranes discharged from the uterus after the birth of offspring, which might be why the title screen is bloody.